阿拉伯聚糖检测试剂盒 产品货号：K-ARAB 　

阿拉伯聚糖检测试剂盒 产品货号：K-ARAB 　

产品货号：K-ARAB 

品牌：Megazyme
产品品名：阿拉伯聚糖检测试剂盒
英文品名：Arabinan Assay Kit
规格型号：100 assays per kit   

For the measurement of arabinan in fruit juice concentrates.

上海西宝生物专业提供阿拉伯聚糖检测试剂盒，原装进口，咨询热线：400-021-8151

INTRODUCTION:
In the processing of apples and pears, the yield of juice can be dramatically improved by using enzymes to degrade the pulp polysaccharides, together with more exhaustive extraction of the pulp with diffusion equipment. Of course, these processes also significantly increase the amount of partially degraded polysaccharide which is solubilised. This polysaccharide material may be soluble as extracted, but subsequent changes in temperature and pH conditions can directly lead to precipitation (or crystallisation) or to chemical modification followed by precipitation. Such a problem can be experienced in the production of clear apple or pear juice, in which case, an arabinan haze material is produced. This material was shown to be microcrystalline 1,5-α-L-arabinan. Arabinans, as present in cell-wall pectic-substances, have been shown to consist of a main chain of 1,5-α-linked L-arabinofuranosyl residues to which other L-arabinofuranosyl residues are linked 1,3-α and 1,2-α in either a comb-like or a ramified arrangement. It is generally accepted that the best solution to this “arabinan haze” problem is to use pectinase enzyme preparations containing high levels of both α-L-arabinofuranosidase and endo-1,5-α-Larabinanase. The combined action of these two enzymes reduces arabinan to arabinose and lower degree of polymerisation, arabinooligosaccharides.
KITS:
Kits suitable for performing 100 assays are available from Megazyme.The kits contain the full assay method plus:
Bottle 1: Buffer (25 mL, pH 8.6) plus sodium azide (0.02 % w/v) as a preservative (Buffer A).Stable for > 2 years at 4°C.
Bottle 2: NAD+.Stable for > 5 years at -20°C.
Bottle 3: α-L-Arabinofuranosidase plus endo-arabinanase suspension (2.2 mL).Stable for > 4 years at 4°C.
Bottle 4: β-Galactose dehydrogenase plus galactose mutarotase suspension, 2.2 mL. Stable for > 2 years at 4°C.
Bottle 5: L-Arabinose standard solution (5 mL, 0.5 mg/mL in 0.02 % sodium azide).Stable for > 4 years at 4°C.
Bottle 6: Arabinan control in mannitol: Arabinan concentration on vial. Stable for > 5 years at 4°C.

Component 7: Pharmacia PD-10 column.

 

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